Fig. 9. Effects of DNMT1 inhibition on suppressing the metastasis of GC cells. (A) DNMT1 siRNA decreased DNMT1 expression in NCI-N87 cells dose dependently. (B) Wound healing assay of NCI-N87 before and after DNMT1 transcription decreased by SiRNA and inhibited by 5-Aza-dC. Histograms showed the ratio of migrated cells to the denuded zone of PepE treated cells when compared with control. (C) Migration and invasion assays of NCI-N87 before and after DNMT1 transcription decreased by SiRNA and inhibited by 5-Aza-dC. Histograms showed the ratio of migration cells and invasion cells of SiRNA and 5-Aza-dC group when compared with control. Cell numbers were counted in five randomly selected microscopic fields. All the above data is shown as mean ± SD of three independent experiments. *P<0.05; **P<0.01.